Highlights
- •The miR-93 levels in plasma and neutrophil were evidently reduced in AIS patients.
- •Neutrophil miR-93 levels were positively correlated with the Barthel Index 7 days after stroke.
- •MiR-93 levels in plasma and neutrophil of AIS patients were negatively correlated with the expression of TNF-α and IL-10.
- •MiR-93 treatment decreased the OGD–induced proliferation of BV2 microglial cells.
Abstract
The present study evaluated the diagnostic and predictive potential of microRNA-93
in acute ischemic stroke (AIS) patients within 6 h of stroke onset and its regulation
on microglial inflammation in vitro. Our results showed that the miR-93 levels in
plasma and neutrophil detected by real-time PCR were evidently reduced in AIS patients,
and Pearson's correlation analysis showed that miR-93 levels in plasma and neutrophils
had a significant positive linear correlation. Moreover, miR-93 levels in plasma and
neutrophils of stroke patients at time of admission were not correlated with infarct
volume and NIHSS (National Institute of Health stroke scale) scores at admission,
but neutrophil miR-93 levels were positively correlated with the Barthel Index 7 days
after stroke. Importantly, miR-93 levels in plasma and neutrophil of AIS patients
were negatively correlated with the expression of TNF-α and IL-10. Furthermore, in vitro treatment with miR-93 agomir decreased the OGD (Oxygen and glucose deprivation)–induced
proliferation of BV2 microglial cells tested by Flow cytometry. We demonstrated that
miR-93 in blood has a potential to facilitate the diagnosis and prediction of neurological
outcomes of acute ischemic stroke, and is involved in inflammation possibly through
targeting the proliferation of microglia.
Keywords
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Article info
Publication history
Published online: December 19, 2018
Accepted:
December 11,
2018
Received:
January 11,
2018
Identification
Copyright
© 2018 Elsevier Ltd. All rights reserved.
