Summary
Background
Choline (Cho) containing compounds are usually evaluated using magnetic resonance
spectroscopy (MRS) by relative ratios such as Cho/N-acetylaspartate (NAA) and Cho/creatine (Cre) ratios. To clarify the significance
of Cho level in gliomas, we evaluated the quantified Cho level using MRS and compared
it with the proliferation activity as determined by MIB-1 immunoreactivity in the
histological specimen.
Methods
There were seven benign and seven malignant gliomas. MRS was obtained using a single-voxel
proton regional imaging of metabolites (PRIME) sequence with three different TE for
T2 compensation. Quantified Cho level was compared with the number of MIB-1 immunopositive
positive cells and cell density in surgical specimens.
Result
A positive correlation was observed between Cho and MIB-1 in benign gliomas, whereas
there was a trend to an inverse correlation in malignant gliomas. This inverse correlation
became a positive correlation when the necrotic area of the tumor (on the T1-weighted
gadolinium enhanced images) was excluded from the voxel of interest (VOI) for MRS,
but this correlation did not reach statistical significance.
Conclusions
The quantification data clarified the behavior of Cho in malignant gliomas. The quantification
method has the advantage of limiting the influence of other metabolites on Cho determination.
In particular, the levels of other commonly measured metabolites, including Cre, may
also be altered in glioma, making ratios between metabolites misleading. Heterogeneity
in the MRS VOI should be considered when evaluating the proliferative activity of
malignant glioma by MRS.
Keywords
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Article info
Publication history
Accepted:
August 3,
2004
Received:
February 2,
2004
Footnotes
This research was supported in part by a research grant-in-aid for special research projects from the University of Tsukuba, awarded to A. Matsumura.
Identification
Copyright
© 2005 Elsevier Ltd. Published by Elsevier Inc. All rights reserved.
